This list represents the comprehensive set of hormones, metabolites, and markers measured in the DUTCH Plus test, which stands for Dried Urine Test for Comprehensive Hormones. 

At Onelife Home Healthcare, Dubai, you can conveniently book the Dutch Plus Test at home . Our DHA-certified team ensures a safe sample collection and accurate reporting from trusted laboratories — without you having to visit a clinic.

The test uses dried urine and saliva samples to provide an in-depth assessment of:

• Sex hormones (Estrogens, Progesterone, Androgens) and their metabolic pathways.

• Adrenal hormones (Cortisol and Cortisone) and their daily rhythm (using saliva) and overall production/clearance (using urine metabolites).

• Neurotransmitter metabolites, oxidative stress markers, and certain nutritional markers.

Key Categories and What They Indicate

1. Progesterone Metabolites (Urine)

• b-Pregnanediol & a-Pregnanediol: These are the primary inactive metabolites of progesterone. Their levels reflect the total progesterone production in the body.

2. Estrogens and Metabolites (Urine)

• Estrone (E1), Estradiol (E2), Estriol (E3): The three main forms of estrogen. E2 is the most potent.

• 2-OH-E1/E2, 4-OH-E1/E2, 16-OH-E1: These are estrogen metabolites. Their ratios are crucial as they show how the body is detoxifying and clearing estrogens. The 2- and 4-OH pathways are generally considered "safer" than the 16-OH pathway.

• 2-Methoxy-E1/E2: Further detoxification products, indicating the methylation activity, a key step in estrogen clearance.

• Total Estrogen: The sum of E1, E2, and E3.

3. Androgens and Metabolites (Urine)

• DHEA-S (Dehydroepiandrosterone Sulfate): A key precursor hormone for both testosterone and estrogen.

• Testosterone & Epi-Testosterone: The primary male sex hormone and its isomer.

• Androsterone & Etiocholanolone: Metabolites of DHEA and testosterone, reflecting the overall androgen production.

• 5a-DHT, 5a-Androstanediol, 5b-Androstanediol: These indicate the activity of the 5-alpha reductase enzyme, which converts testosterone to the more potent Dihydrotestosterone (DHT). The ratio of 5a to 5b metabolites is important for assessing androgen activity.

4. Free Cortisol and Cortisone (Saliva)

• Saliva Cortisol/Cortisone - Waking, +30 min, +60 min, Afternoon, Night: This measures the free, biologically active fraction of cortisol (the "stress hormone") and cortisone throughout the day. This provides a Cortisol Awakening Response (CAR) reading and the diurnal rhythm, which is vital for assessing adrenal health and HPA axis function.

• Saliva Cortisol/Cortisone Total: The sum of the measured points.

• *Saliva Cortisol/Cortisone - Insomnia: An extra measurement to assess hormone levels during a sleep disturbance.

5. Cortisol Metabolites and DHEA-S (Urine)

• a-Tetrahydrocortisol (a-THF), b-Tetrahydrocortisol (b-THF), b-Tetrahydrocortisone (b-THE): These are the metabolites of cortisol and cortisone.

• Metabolized Cortisol (THF+THE): This sum reflects the total cortisol production over 24 hours and how quickly the liver is clearing or metabolizing cortisol. This is a crucial distinction from the free cortisol measured in saliva.

6. Additional Markers

• Melatonin (6-OH-Melatonin-Sulfate):* Reflects the body's sleep hormone production.

• Oxidative Stress / DNA Damage (8-OHdG): A marker that assesses damage to DNA caused by free radicals, an indicator of overall oxidative stress.

• Neurotransmitter Metabolites:

  • Homovanillate (HVA): Metabolite of Dopamine.

  • Vanilmandelate (VMA): Metabolite of Norepinephrine and Epinephrine (Adrenaline).

• Vitamin Markers:

  • Methylmalonate (MMA): A functional marker for Vitamin B12 status.

  • Xanthurenate, Kynurenate: Markers for Vitamin B6 status.

• Glutathione Marker (Pyroglutamate): A marker that can reflect the status of glutathione, the body's master antioxidant.

• Creatinine (Urine): Measured as a control to ensure the urine samples are not too dilute or concentrated, helping to normalize the hormone results.